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Research Assistant

£39,878 - £39,878
 

Job Description

This post will be based in the Immunology Group headed by Mr Wayel Jassam and Dr Yun Ma at the Institute of Liver Studies (ILS) at the King’s College Hospital and King’s College London, Denmark Hill campus. The post holder will be focused on two related projects: 




  1. To perform liver transplantation related research project entitled: Assessing the effect of Andrographolide (Andro) on fatty liver graft in the setting of ex-situ normothermic machine perfusion: Andro is a botanical compound which has a potent anti-inflammatory property by inhibiting NF-κB, Nrf2 activation and in reducing fat content of liver in animal model. The candidate will collect hepatocytes either coming from fatty liver draft or normal liver (leftover from split liver transplant) to set up cell culture after adding different doses of Andro. The effect of Andro on fatty liver hepatocytes or normal will be accessed by several aspects: 1) cell viability; 2) fat content change; 3) mitochondria function; 4) on gene expression by RNA-seq, ChIP-seq analyses etc.

  2. To construct liver organoid as a 3D model to mimic live liver, the assess will be performed similar to those in vivo.  Liver organoid will be constructed using either fatty liver graft or normal liver graft.



The candidate will also be involved in the training of BSc students who are accepted in the current summer vacation for receiving basic training on Immunologic technique



This post is a fixed-term contract with the possibility to further extension, being a part-time post: 20 hours per week for five weeks.




Qualifications

  • Medical Qualified or equivalent level in Immunology, including transplantation immunology and tumour immunology

  • Expertise in blood processing, liver tissue processing, T cell and B cell immunology in human beings

  • Willingness to work as part of a team, to collaborate and help other colleagues when they need

  • Excellent verbal and written communication skills



Skills

  • Having performed or mastered following techniques: 1) constructing organoid; 2) molecular biology techniques (RNA isolation etc). 3) multiple parameter flow cytometry experimental setup and analysis

  • Hepatocyte isolation and culture

  • Statistical analysis of data

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Project Overview: We are developing a web application and are seeking an experienced Backend Developer to manage API development, cloud deployment, and database management. This project operates within an Agile/Sprint framework. 

 

Key Responsibilities:

  • Python Proficiency: Develop backend logic using Python, with a strong focus on class structures and JSON manipulation.
  • Flask API Development: Create and manage APIs using Flask, including developing POST/GET endpoints and structuring backend repositories.
  • Session Management: Implement JWT authentication for session management, integrated with frontend components.
  • Cloud Deployment: Deploy backend services on AWS EC2, including the use of Linux command line, Nginx reverse proxy, and PM2 for process management.
  • Frontend Deployment: Manage the deployment of frontend applications on AWS S3.
  • AWS Services: Utilize AWS Route 53 for DNS management, AWS Certificate Manager for SSL/TLS, and invalidate AWS CloudFront distribution caches as needed.

 

As a member of our team, you will be expected to follow best practices, including code decoupling, maintaining cohesion, thorough manual testing, clear naming conventions, and proper use of Git (e.g., avoiding pushing directly to the main branch).

 

Database Management:

  • MongoDB Atlas: Manage and manipulate MongoDB Atlas (NoSQL) databases using PyMongo.
  • Data Aggregation: Create and manage data aggregation pipelines to support database operations.

 

Benefits of Joining Our Team:

  • Be part of a startup-style project within King?s College London, working on a cutting-edge web application alongside a team of professors and students. Contribute to a project that has received multiple rounds of funding and awards. 
  • Develop critical skills in project management, communication, and teamwork, all while working in a collaborative and supportive environment.
  • Gain access to King?s College London internal events, where you can network with academics, industry leaders, and other like-minded students.


Qualifications

How to Apply: Please submit your resume and a portfolio of your work demonstrating relevant experience.



Skills

Experience & Expectations:

  • Experience Required: Previous experience in the mentioned technologies and tasks is preferred. If lacking, a strong willingness to learn independently and contribute effectively is crucial.
  • Punctuality: Adherence to project timelines and deadlines is essential.
  • Work Ethic: Demonstrated work ethic could lead to extended contracts. 

 

Research assistant required to work on a project that investigates the higher education experiences and outcomes for mixed-heritage/raced young people (18-25yrs) in London. The project is a mixed-method study consisting of quantitative data analysis of existing public survey datasets, focus groups with mixed-heritage students in London universities, sixth-forms and colleges, and semi-structured interviews with school and university staff. The study is led Dr Ayo Mansaray (School of Education, Communication and Society) with Dr Chiamaka Nwosu (King?s Business School).

The post-holder will provide research support for the qualitative elements of the project, including tasks such as: recruitment and arrangement of student and staff interviews, contributing to the drafting of qualitative interview topic guides, conducting student focus groups and one-to-one staff interviews, data management (e.g. cataloguing data files, sending files for transcription etc.), data analysis, and report writing. The RA will also provide ad hoc administrative support to the project team. The RA will be expected to attend in-person project meetings with funders in London.



Qualifications

BA and MA/MSC in a Social Science/Arts & Humanities discipline, undertaking a doctorate.



Skills

Experience of MS Teams, and MS office suite (e.g. Word etc.),  and knowledge of bibliographic software (e.g. Endnote) and qualitative data analysis software (e.g. Nvivo). 

Interest in the experiences of racialised groups in the education system and/or social justice in education. 

Understanding of research design and project management. Good understanding of qualitative methodologies, specifically prior experience of focus group or individual interviewing. 

Good communication, organisational and interpersonal skills. 

Ability to travel across London to attend meetings and conduct interviews. 

We are looking for an enthusiastic technician to join our team of scientists and clinicians to develop bone marrow organoids and investigate the interaction between malignant cells and the immune system. The successful candidate will work with fresh and frozen peripheral blood and bone marrow samples from healthy donors and patients with myeloproliferative neoplasm. 

The Research technician needs to use a variety of techniques such as flow cytometry, cell culture, imaging and other necessary techniques. 



Qualifications

A degree in biology or an equivalent degree in a relevant subject.



Skills

Essential:

Experience in cell culture, flow cytometry, and work with organoids.

Familiar with essential molecular techniques.

 

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